Timokinonun Perinatal Sıçan Beyninde Valproik Asit İndüklü Oksidatif Stres Üzerine Etkileri

Thymoquinone (TQ), bioactive molecule of black cumin, has antioxidant and neuroprotective effects. TQ’s hypoglecemic effect while applied prenatally is reported. This study aimed to find the TQ dose with maximum minimum side effects in valproic acid (VPA) induced oxidative stress. Pregnant Wistar rats were injected i.p. 400 mg/kg/ml VPA on embryonic day 12.5 (E12.5). Repeated groups from E11.5- E14.5; RC- repeated control: did not receive TQ, R1: 0.5 R2: 2 R3: 4 R4: 8 TQ. Single E12.5; SC- single S1: S2: 15 Pups sacrificed postnatal 7. Glutathione (GSH), malondialdehyde (MDA) superoxide dismutase (SOD) levels measured via ELISA method. Prenatal exposure decreased GSH SOD RC SC compared naïve group. R3 group showed improved RC. No significant difference MDA was found between groups. Antioxidant stress been can be used investigate other parameters that are affected by prenatal exposure.